由于環(huán)境因素和細胞內的正常代謝過程造成的DNA損傷，每個細胞每天都會發(fā)生1,000到1,000,000個。雖然這些只占人類基因組約60億個堿基中的一小部分，但如果關鍵基因損傷未及時修復，可能會阻礙細胞的正常生理功能，進而增加癌變可能。彗星實驗，或稱單細胞凝膠電泳（Single cell gel electrophoresis，SCGE），是一種測量單個細胞DNA損傷的常用技術。其原理很簡單，即在電泳場中，將受損細胞DNA（包含片段和鏈斷裂）與完整的DNA分離，通過顯微鏡可觀察到損傷細胞呈現出典型的彗星狀尾巴，然后通過測量計算彗尾大小對比出細胞DNA損傷的程度。因為彗星實驗的特點，該方法幾乎被用來評估任何類型的真核細胞的 DNA 修復能力，包括雙、單鏈斷裂的不同的 DNA 損傷情況。是一種能快速、大通量檢測真核細胞DNA損傷進而判別遺傳毒性的技術。

彗星實驗結果圖

彗星實驗原理雖簡單，但操作繁瑣，需要豐富的實驗經驗和技巧，尤其常常出現的“脫膠”問題，困擾了許多科研人員。除此之外，有時為了跑出完美的“彗星”圖案放在paper里，還需要重復做許多次實驗，費時費力。為了解決上述問題，我們推薦CellBiolabs的OxiSelectTMComet Assay Kit即彗星實驗試劑盒來檢測細胞的DNA損傷。該試劑盒不僅能讓彗星實驗化繁為簡，還有兩種不同規(guī)格（3孔和96孔）的細胞電泳凝膠板供選擇，讓少量樣本和大量樣本的DNA損傷檢測通通輕松hold住。用該試劑盒做彗星實驗流程如下圖。

除了操作簡便，OxiSelectTMComet Assay Kit還有以下優(yōu)點：

1)      適用于各種DNA損傷檢測，是一款非常好用的DNA損傷檢測篩選工具；

2)      試劑盒中的載玻片經過特殊處理以粘附低熔點瓊脂糖，避免“脫膠”問題出現；

3)      采用特殊的DNA熒光染料，能有效降低背景干擾，更加方便讀取實驗結果。

彗星實驗試劑盒信息：

為了滿足客戶更多樣的實驗需求，彗星實驗試劑盒內特殊處理電泳載玻片還可以單獨購買，詳情如下：

產品部分發(fā)表文獻：

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